We are offering RT-PCR tests for the detection and/or quantitation of a wide range of viruses, bacteria and gene mutations. All the tests are highly automated resulting in reduced turn-around time for reports. RT-PCR technology is currently a powerful tool in molecular diagnostics. In viral, bacterial and parasitical detection, quantification and typing, the classical detection methods such as culture and immunoassays have been replaced or supplemented by this technology. By direct detection of DNA or RNA of the pathogen, RT PCR allows for an early and highly specific diagnosis, thus leading to a fast and selective treatment regime of the individual patient.

● Hepatitis B Virus (HBV)
● Hepatitis C Virus (HCV)
● HIV type 1 (HIV-1)
● Enterovirus
● Adenovirus
● Bordetella pertussis/parapertussis
● Chlamydia pneumoniae
● Legionella pneumophila
● Mycoplasma pneumoniae
● Mycobacterium tuberculosis

Several viral infections have been shown to occur in immunocompromised hosts like tumour bearing hosts, transplant recipients and human immunodeficiency virus (HIV) infected individuals. In these categories of patients they cause severe morbidity and disseminated fatal infections if untreated. Early and specific detection of opportunistic viruses is possible today by using certain new techniques like real-time multiplex polymerase chain reaction.

● Cytomegalovirus (CMV)
● Epstein-Barr Virus (EBV)
● BK/JC Virus (BK/JC)
● Adenovirus
● Aspergillus
● Herpes Simplex Virus (HSV-1/2)
● Human Herpesvirus 6/7 (HHV-6/7)
● Human Herpesvirus 8 (HHV-8)

Genetic disorders diagnosis is very important and thus to ensure an accurate result, it requires the highest standards in laboratory practice. Real-time PCR offers more rapid and potentially higher throughput assays, without compromising accuracy and a wide dynamic range of detection with a high degree of sensitivity. Therefore, using this hi tech method genetic diagnosis can be diagnosed with better precision and sensitivity. FII Prothrombin.

● FV Leiden
● FV Cambridge
● FV R2 (H1299R)
● FXIII
● MTHFR
● FV R2 (H1299R)
● MTHFR 677
● PAI 4G/5G
● CF
● SMA
● Beta Thalassemia
● Connexin
● Hemochromatosis
● Warfarin
● Y Chromosome 8 Microdeletion
● Chromosome 10 Microdeletion
● Y Chromosome 15 Microdeletion
● Jak-2
● HLA B51
● HLA B52
● HLA B27
● FMF

Sexually transmitted diseases (STD) are a major cause of infertility, long-term disability, ectopic pregnancy, and premature birth. They increase the risk of developing genital cancers, and are a severe medical, social, and economic burden to thousands of adults and babies worldwide. To date, more than 30 pathogens such as bacteria, viruses, and parasites have been shown to be transmitted through sexual contact. Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis, Mycoplasma hominis, Ureaplasma urealyticum, Ureaplasma parvum, herpes simplex virus (HSV) are reportedly the most prevalent STD pathogens. Novel multiplex RT-PCR test for STD is the molecular diagnostics tool with better sensitivity, specificity and reproducibility.<

● Chlamydia trachomatis (Chlamydiasis)
● Neisseria gonorrhoeae (Gonorrhea)
● CT/NG/MG Multiplex
● MH/UU/UP Multiplex
● Mycoplasma genitalium/hominis
● Herpes Simplex Virus (HSV-1/2)
● Trichomonas vaginalis
● Human Papillomavirus (HPV)

Gastrointestinal infections in both pediatric and adult patients account for significant morbidity and mortality worldwide. In addition, immunocompromised hosts, including HIV patients, solid-organ transplant recipients or patients requiring therapy for chronic inflammatory diseases are susceptible to gastrointestinal infections. This can be caused by a number of pathogens including viruses, bacteria, and parasites. Presentations of gastroenteritis with an unidentified source pose a challenge to health care providers, as the same clinical presentation can be caused by different etiologies. Conventional detection methods e.g. electron microscopy and immunoassays apparently lack sufficient sensitivity. RT-PCR helps to diagnose this with higher sensitivity and specificity.

● GIST Mutation (PDGFRA somatic mutations)

The molecular diagnosis of a wide range of different neurological syndromes was established by a reverse transcription multiplex PCR assay. The applications of RT multiplex PCR assay are extensive and prove to be particularly valuable for the rapid and sensitive diagnosis of neurological diseases in both immunocompetent and immunocompromised patients.

● Borrelia burgdorferi
● Enterovirus
● Herpes Simplex Virus (HSV-1/2)
● Varicella-Zoster Virus (VZV)

Multiplex PCR can be used to detect several resistance markers at once facilitating identification of multi-drug-resistant organism.

● MRSA
● VRE

Molecular Diagnostics based on RT-PCR can be used to provide accurate prognosis and to predict response, resistance, and toxicity to cancer therapy.
We endeavour to optimise RT-PCR techniques for the detection of minimal residual disease(MDR), single nucleotide polymorphisms and chromosomal translocations. In numerous haematological malignancies the detection of MRD directly correlates with the clinical outcome of the disease. Proving that MRD monitoring is important for therapy guidance in clinical settings. RT-PCR based techniques are used in clinical protocols for the detection of MRD.
Our in-house research team endeavours for the integration of molecular markers into existing histomorphologic classifications in surgical pathology to provide additional stratification for a more accurate prognosis. Believing that recent advances in molecular definition of cancer may allow them to develop new kits for the guidance of therapy and the ability to monitor residual disease.

Cancer:

● KRAS Mutation
● KRAS/BRAF Mutation Analysis (KRAS exons 2, 3, 4 & BRAFV600E)
● NRAS Mutation
● RAS Mutation Screening Panel (KRAS & NRAS exons 2, 3, 4)
● Colorectal Cancer Mutation Detection Panel
● PIK3CA Mutation Detection
● Cell-Free RAS Mutation Detection

Lung Cancer:

● EGFR Mutation Analysis
● PIK3CA Mutation Detection (exons 9 and 20)
● Cell-free EGFR Mutation Detection
● Lung Cancer RNA Panel (ALK, ROS1, RET, MET exon 14 skipping)

Breast Cancer:

● PIK3CA Mutation Detection
● BRCA Complete™ BRCA-NGS

Leukemia:

● BCR-ABL P210 (Mbcr) One-Step Detection
● BCR-ABL P190 (mbcr) One-Step Detection
● Leukemia Translocation Panel
● AML1-ETO One-Step Detection
● E2A-PBX1 One-Step detection
● MLL-AF4 One-Step Detection
● PML-RARA bcr1,2,3 One-Step Detection
● TEL-AML1 One-Step Detection
● CBFb-MYH11 One-Step Detection

Melanoma:

● BRAF Mutation Analysis (V600E/K/D/R/M/G)
● NRAS Mutation Analysis (exons 2, 3, 4)
● Cell-free BRAF Mutation Detection

Thyroid Cancer:

● Thyroid Cancer Mutation Detection (BRAF V600E, KRAS codon 12/13, NRAS codon 61, HRAS codon12/13/61)
● Thyroid Cancer Fusion Gene Detection (RET/PTC1, RET/PTC3, PAX8/PPARγ)

Giloblastoma:

● MGMT Methylation Detection
● IDH1/2 Mutation Detection
● EGFR V3 Detection